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Campy DNA
tm3130612277
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Campylobacter jejuni causes 5-14% of all diarrhoeal diseases worlwide especially among children. Current diagnosis of the disease is via culture and Polymerase Chain Reaction (PCR) method. The culture method is time consuming, laborious, low in sensitivity and is being replaced by PCR-based tests. PCR is one of the most common DNA-based techniques used for the detection and identification of pathogenic microorganisms. Speed, specificity and sensitivity are among its important advantages. Limitations of PCR-based tests include reagents that must be transported in cold chain, it is expensive and requires skilled personnel to perform the test. Furthermore, multiple pipetting and repeated freeze-thawing of PCR reagents can give erroneous results.

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Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia
16150 Kubang Kerian
Kelantan
Malaysia

Full Name [1]
Prof Asma Ismail
Email [1]
asma@kb.usm.my