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EZ Dysentery DNA
tm3129612267
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PCR (Polymerase Chain Reaction) is one of the common DNA-based technique used for detection and identification of pathogenic microorganisms. Speed, a good detection limit, selectivity, specificity, sensitivity and potential for automation are among its important advantages. The main limitation of PCR based tests is its reagents has be transported by cold storage and repeated freeze thawing and multipipeting of the reagents can give erroneous results.

Unlike the conventional PCR based test, the EZ Dysentery DNA kit contains thermostabilized PCR reagents in a single tube to detect for Shigella sonnei, Shigella flexneri and Shigella dysentery respectively within 3 hours. The test can be transported without cold chain and is simple to perform. When evaluated in a retrospective study, the test showed 100% sensitivity and specificity for the detection of S. flexneri and S. dysentery and 100% sensitivity and 97.8% specificity for S.sonnei.

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Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia
16150 Kubang Kerian
Kelantan
Malaysia

Full Name [1]
Prof Asma Ismail
Email [1]
asma@kb.usm.my