This invention to produce Dental Sponge as a haemosthatic agent for use in dentistry. Extraction of teeth causes bleeding and resorption of alveolar bone. Bleeding after extraction of teeth should be stopped before the patient is discharged. A haemosthatic agent is often used in cases of profuse bleeding or prolonged bleeding. In this situation a good haemosthatic agent is very useful. Commercial haemosthatic agents available in our market are expensive and most of them are produced from animal gelatin and therefore not acceptable for some segment of community.
Dental sponge was prepared from local calf bone and underwent a through processing techniques. Endotoxin level assay was determined, surface properties were investigated in pore sizes and water uptake ability were measured. Biological evaluation was carried out testing for its cytotoxicity on human fibroblast cell lines. In vivo studies were done on sheep model. Bleeding time was evaluated in extraction sockets of sheep and the alveolar bone height was measured before and 2 months post-extraction in a case control study. There was a statistically significant difference in haemostatic effect showing shorter bleeding time in group using dental sponge compared to its control.