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Typhi Carrier DNA
tm3127312244
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Typhoid is a major public health problem. It is caused by the bacteria Salmonella typhi which is transmitted via food prepared by food handlers who are carriers. The current method for diagnosis of typhoid carriers is by stool culture which produces results within 2-7 days, has low isolation rate (2-5%), it is labour intensive and expensive since multiple stool cultures need to be performed. Hence, there is rapid, specific, sensitive, cost effective, simple to perform and without need for cold chain for transportion and storage. Polymerase Chain Reaction (PCR) is one of the most common DNA-based techniques used for microbial diagnostics. Although sensitive, specific and rapid its reagents have to be transported via cold storage. Also, repeated freeze-thawing and multiple pipetting of reafents can give erroneous results.

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Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia
16150 Kubang Kerian
Kelantan
Malaysia

Full Name [1]
Prof Asma Ismail
Email [1]
asma@kb.usm.my