Enterococci have emerged as prominent nosocomial pathogens that cause clinical infections, such as urinary tract infection, surgical wound infection, bacteremia and endocarditis. Ever the last decade, Centre for Disease Control (2000) reported a dramatic increase in the occurrence of vancomycin resistant enterococci (VRE) in hospitals within the United Kingdom and the United States (Stephan et al. 2002), Australia, Belgium, Canada, Denmark, Germany, Italy, The Netherlands, Spain and Sweeden (Mayhall et al, 2000). However, sporadic cases have been reported in poultry samples in Malaysia (Shah-Majid et al, 2004), Where in there is a high risk of transmission of resistant strains to humans during handling or consumption of poultry meat. This problem necessitates an urgent need to develope a diagnostic kit for rapid and accurate identification of Enterococcus species and its antibiotic-resistant genotypes to facilitate surveillance of VRE in poultry and human samples.