Hybridization is interbreeding between two animals or plants of different taxa. The increase in the level of production traits due to acrossing is called hybrid vigour or heterosis. The term hybrid vigour points at the vigour, which is a real factor that can be seen with the naked eye, in comparison to pure breeding. Seed production of hybrid groupers is mainly about manipulating good characteristics of brood stock for stock improvement. Our research focuses on four grouper species specifically Tiger grouper- Epinephelus fuscoguttatus; Orange-spotted grouper- Epinephelus coioides, Giant grouper- Epinephelus lanceolatus and Mouse grouper- Cromleptes altivelis.